Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis | Zendy

Gardiner Chris | Zendy; Ferreira Yannick J. | Zendy; Dragovic Rebecca A. | Zendy; Redman Christopher W.G. | Zendy; Sargent Ian L. | Zendy

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Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis

Author(s) -

Gardiner Chris,

Ferreira Yannick J.,

Dragovic Rebecca A.,

Redman Christopher W.G.,

Sargent Ian L.

Publication year - 2013

Publication title -

journal of extracellular vesicles

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 5.94

H-Index - 68

ISSN - 2001-3078

DOI - 10.3402/jev.v2i0.19671

Subject(s) - nanoparticle tracking analysis , extracellular vesicles , enumeration , sizing , extracellular vesicle , protocol (science) , microvesicles , computer science , tracking (education) , vesicle , nanoparticle , extracellular , nanotechnology , chemistry , computational biology , materials science , mathematics , biology , microbiology and biotechnology , membrane , biochemistry , medicine , pathology , gene , microrna , pedagogy , alternative medicine , organic chemistry , combinatorics , psychology

Nanoparticle tracking analysis (NTA) is a light‐scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration of NTA measurements and describe in detail a protocol for the analysis of EVs by NTA which should minimise many of the sources of variability and imprecision associated with this technique.

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