Open AccessExtracellular vesicle sizing and enumeration by nanoparticle tracking analysis
Author(s) -
Gardiner Chris,
Ferreira Yannick J.,
Dragovic Rebecca A.,
Redman Christopher W.G.,
Sargent Ian L.
Publication year - 2013
Publication title -
journal of extracellular vesicles
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.94
H-Index - 68
ISSN - 2001-3078
DOI - 10.3402/jev.v2i0.19671
Subject(s) - nanoparticle tracking analysis , extracellular vesicles , enumeration , sizing , extracellular vesicle , protocol (science) , microvesicles , computer science , tracking (education) , vesicle , nanoparticle , nanotechnology , chemistry , biological system , materials science , mathematics , biology , microbiology and biotechnology , medicine , pathology , biochemistry , membrane , microrna , gene , combinatorics , psychology , pedagogy , alternative medicine , organic chemistry
Nanoparticle tracking analysis (NTA) is a light‐scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration of NTA measurements and describe in detail a protocol for the analysis of EVs by NTA which should minimise many of the sources of variability and imprecision associated with this technique.