Open AccessGenetic Diversity and Population Structure of Endangered Catfish Rita rita (Hamilton, 1822) Revealed by Heterologous DNA Microsatellite Markers
Author(s) -
Muhammad Forhad Ali,
AUTHOR_ID,
Md. Rafiqul Islam Sarder,
Mohammad Matiur Rahman,
M.F.A. Mollah,
Md. Abdus Salam
Publication year - 2021
Publication title -
asian fisheries science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.275
H-Index - 7
eISSN - 2073-3720
pISSN - 0116-6514
DOI - 10.33997/j.afs.2021.34.2.007
Subject(s) - biology , genetic diversity , microsatellite , population , allele , catfish , gene flow , genetics , endangered species , locus (genetics) , genetic variation , zoology , ecology , gene , fishery , demography , sociology , fish <actinopterygii> , habitat
Genetic information is essential for conservation and future aquaculture development of the endangered catfish Rita rita (Hamilton, 1822). Two hundred catfish, R. rita, 50 from four rivers, the Old Brahmaputra, Jamuna, Meghna and Kangsa were collected and analysed to evaluate the genetic diversity and population structure using five microsatellite primers (Cba06-KUL, Cba08-KUL, Cba09-KUL, Phy03-KUL and Phy07-KUL). Four of the five amplified loci were found polymorphic (P95) in all the populations and 46 alleles were recorded with 9 to 14 alleles per locus. Differences were observed in the total number of alleles ranging from 41 to 44, effective number of alleles from 29.96 to 37.46, observed heterozygosity from 0.57 to 0.76, Shannon’s information index from 2.09 to 2.30 and polymorphic information content from 0.84 to 0.88 among the four populations. Results exposed the highest levels of genetic diversity in the Meghna population while the lowest in the Kangsa population of R. rita. All the populations were significantly deviated (P < 0.001) from the Hardy-Weinberg equilibrium for all the loci. Nei’s genetic distance between populations ranged 0.007 to 0.017 with low overall genetic difference FST = 0.011 and high gene flow Nm = 24.333, indicating that R. rita populations were not subdivided. This study revealed a high level of gene diversity with deficiency in genetic heterogeneity in all the populations of R. rita, emphasising natural management, conservation and rehabilitation measures of this species.