Open AccessIn vitro culture of Nonea pulla DC. seeds.
Author(s) -
Е. Ф. Семенова,
K.V. Vedernikova,
E.Yu. Schetneva
Publication year - 2020
Publication title -
proceedings of v international scientific conference “current state, problems and prospects of the development of agrarian science”
Language(s) - English
Resource type - Conference proceedings
DOI - 10.33952/2542-0720-2020-5-9-10-95
Subject(s) - germination , in vitro , horticulture , biology , botany , tissue culture , agronomy , biochemistry
Nonea pulla DC. is a promising perennial medicinal plant growing in the Crimea. Controlled in vitro cultivation of nonea seeds allows improving the up-to-date techniques of seedlings preparation. The conducted experiments confirmed the low germinating capacity of seeds (5–9 %). To increase this parameter and to speed up the introduction process, we investigated the Nonea pulla in vitro culture. The initial phases of germination were expectedly observed during seeds cultivation. The seed swelling, rupture of pericarp and seed hull, release of germ with cotyledons, dehiscence of cotyledons were detected. Moreover, in some cases, no subsequent development was observed. However, normal germs formed in 60% of cases. Seeds also sprouted without the prior cold stratification. For the following growth, plants required a relatively simple culture medium. The maximum development conditions were reached after 1.0–1.5 months of in vitro cultivation (26±2 °С, illuminance of 2000–3000 lux, 16-hour photoperiod).