Open AccessSome aspects of the genomic DNA isolation from lavender plants of different origin.
Author(s) -
M.S. Zagorskaya
Publication year - 2020
Publication title -
proceedings of v international scientific conference “current state, problems and prospects of the development of agrarian science”
Language(s) - English
Resource type - Conference proceedings
DOI - 10.33952/2542-0720-2020-5-9-10-90
Subject(s) - lavender , genomic dna , dna extraction , isolation (microbiology) , dna , extraction (chemistry) , chromatography , microbiology and biotechnology , chemistry , biology , botany , biochemistry , polymerase chain reaction , bioinformatics , gene , essential oil
The method of DNA extraction from lavender plants has some difficulties. Moreover, molecular genetic studies often use not only fresh materials but also microplants from an in vitro culture or frozen material. For all these types of samples, the DNA isolation technique was developed, which is based on the CTAB buffer, but without the use of liquid nitrogen, mercaptoethanol and sodium acetate, which, in turn, simplifies and reduces the cost of the method.