Open AccessOptimization of cultivated strawberry micropropagation using а biogenic silica and green-tea-flavonoids-based mechanocomposite
Author(s) -
Elena Ambros,
Ekaterina Karpova,
Olga Kotsupiy,
Yu.G. Zaytseva,
Elena Trofimova,
Tatyaovikova
Publication year - 2020
Publication title -
proceedings of v international scientific conference “current state, problems and prospects of the development of agrarian science”
Language(s) - English
Resource type - Conference proceedings
DOI - 10.33952/2542-0720-2020-5-9-10-86
Subject(s) - micropropagation , organogenesis , shoot , husk , biology , botany , murashige and skoog medium , horticulture , axillary bud , cultivar , sowing , fragaria , cytokinin , in vitro , chemistry , tissue culture , auxin , biochemistry , gene
For the first time, organogenesis and physiological characteristics of Fragaria ananassa microclones (cvs. ‘Alpha’ and ‘Solnechnaya polyanka’) under the influence of mechanocomposite (MC) based on rice husks amorphous silica and flavonoids of green tea during the multiplication stage in in vitro conditions were studied. The addition of the MC (0.0, 2.5, 5.0 and 10.0 mg·L-1) to the Gamborg- Eveleg’s basal salt medium supplemented with 0.75 mg·L-1 6-benzylaminopurine has shown beneficial action on processes of organogenesis followed by enzymatic, photosynthetic, and hormonal activities of in vitro cultured strawberry plantlets. In both cultivars, the high frequency of proliferation (100 %) and maximum number of axillary shoots increased by 1.8–2.0 times on medium supplemented with 5.0 mg·L-1 MC. The concentrations of 2.5 and 5.0 mg·L-1 MC were optimal for obtaining plantlets with high physiological state in in vitro conditions. The results may be used for the development of production systems for a healthy planting material using biotechnological approaches and recommended for commercial strawberry micropropagation.