Open AccessThe influence of the nutrient medium composition on the induction of calluso- and morphogenesis of Melissa officinalis L.
Author(s) -
O.V. Yakimova,
N.A. Yegorova
Publication year - 2020
Publication title -
proceedings of v international scientific conference “current state, problems and prospects of the development of agrarian science”
Language(s) - English
Resource type - Conference proceedings
DOI - 10.33952/2542-0720-2020-5-9-10-100
Subject(s) - callus , morphogenesis , explant culture , composition (language) , cultivar , botany , chemistry , horticulture , biology , biochemistry , in vitro , gene , art , literature
The features of the calluso- and morphogenesis induction during the cultivation of tissues and organs of Melissa officinalis depending on endogenous and exogenous factors were revealed. The maximum frequency of callus induction (59.5–92.9 %) was noted on the MS medium with 1.0 mg/l 2.4-D and 0.5 mg/l BAP. The induction of morphogenesis from callus was influenced by the composition of the culture medium, the explant type and cultivar. The maximum frequency of morphogenesis induction (20.0–28.0 % depending on the cultivar) from callus was noted on MS culture medium supplemented with 1.0 mg/L NAA and 0.5 mg/L BAP or 1.0 mg/L NAA and 0.5 mg L TDZ.