Open AccessAbility of the soil cellulolytic bacteria to colonize endophytic niche of barley grains
Author(s) -
Olga Sviridova,
N. I. Vorobyov,
Ya.V. Pukhalsky,
О. Н. Курчак,
О. П. Онищук,
В.И. Сафронова,
И. Г. Кузнецова,
V. N. Pishchik
Publication year - 2020
Language(s) - English
Resource type - Conference proceedings
DOI - 10.33952/2542-0720-15.05.2020.20
Subject(s) - microorganism , biology , rhizosphere , bacteria , botany , agronomy , crop , genetics
To identify microorganisms that can penetrate into the endophytic niche of the grain of barley plants, many years of vegetative experiments were conducted on sod-podzolic soil without the use of mineral fertilizers. In the non-growing season, a biological product, consisting of cellulolytic association of bacteria with genotypic passport, decomposed barley straw. Presowing treatment of seeds was not carried out, therefore, during the growing season; local microorganisms decomposing plant residues could be present in the barley rhizosphere. After six years of rotation of barley plants, the microbiological composition of its seed niche was studied. As a result, it was found that in the seeds of barley bacteria are present in an amount of 240 ± 20 CFU/g of grain. Isolated pure cultures of microorganisms were identified as Cellulomonas gelida, Micrococcus luteus and Bacillus licheniformis by the sequence of ITS fragments of 16S rRNA. These types of bacteria were also present in the used biological product. Based on the research conducted, it can be assumed, that permanent cultivation of barley plants and sowing of seeds of the previous year can contribute to the formation of effective microbial and plant biosystems that are resistant to environmental stress.