Open AccessComposition of microbial communities of leached chernozem depending on agricultural crops
Author(s) -
Yu.Yu. Khatuntseva,
И.В. Черепухина,
Nadezhda Gorbunova,
A. I. Gromovik,
Sugar named after A. L. Mazlumov
Publication year - 2020
Language(s) - English
Resource type - Conference proceedings
DOI - 10.33952/2542-0720-15.05.2020.04
Subject(s) - chernozem , humus , mineralization (soil science) , soil fertility , microorganism , agronomy , soil organic matter , chemistry , environmental science , nutrient , soil water , soil science , biology , bacteria , genetics , organic chemistry
A large role in the creation of soil fertility and water-resistant structure, in the processes of humus synthesis and mineralization, as well as in the provision of agricultural plants with elements of mineral nutrition, belongs to soil microorganisms. For leached chernozem, the influence of field crops on the main groups of microorganisms, which are involved in the formation of effective and potential soil fertility, and associated enzymes was established. The purpose of the work is to study the effect of agricultural plants on the structure of the microbial community of the soil and the activity of enzymes involved in the formation of effective and potential soil fertility. Soil samples were taken from the 0-25 cm layer. They differed in anthropogenic load: virgin soil and soil where crops were cultivated. To count the number of soil microorganisms in the crops and their rhizospheres, we used the method of culturing successive dilutions of the soil suspension on selective nutrient media. The catalase activity of the soil was determined by the gasometric method. It is based on measuring the decay rate of hydrogen peroxide when it is interconnected with the soil in terms of the amount of oxygen released. The method for determining phosphatase activity is based on the quantitative calculation of inorganic phosphorus, which is formed by the breakdown of organic phosphorus compounds under the action of phosphatases.