Open AccessFermented Animal Source Protein as Substitution of Fishmeal on Intestinal Microbiota, Immune-Related Cytokines and Resistance to Vibrio mimicus in Freshwater Crayfish (Cherax cainii)
Author(s) -
Muhammad A.B. Siddik,
Ravi Fotedar,
Reaz Chaklader,
Javed Foysal,
Ashfaqun Nahar,
Janet Howieson
Publication year - 2020
Publication title -
frontiers in physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.32
H-Index - 102
ISSN - 1664-042X
DOI - 10.3389/fphys.2019.01635
Subject(s) - crayfish , biology , vibrio , gut flora , immune system , microbiology and biotechnology , bacteria , ecology , immunology , genetics
A feeding trial was carried out to evaluate the effects of substitution of fishmeal (FM) by dietary poultry by-product meal, fermented by Lactobacillus casei and Saccharomyces cerevisiae on growth, intestinal health, microbial composition, immune related cytokines and disease resistance of freshwater crayfish, marron ( Cherax cainii ) against Vibrio mimicus. Two isonitrogenous and isocaloric diets were formulated by replacing FM protein with fermented poultry by-product meal (FPBM) protein at 0% (Control) and 75% (FPBM), and fed marron for 70 days. The results indicated no significant difference ( P > 0.05) in final body weights between two groups of marron, whilst intestinal microvilli number per fold was increased in marron fed FPBM than the control. The 16S rRNA sequences revealed an increased number of Lactobacillus and Streptococcus , and decreased number of Aeromonas at genus level in the distal intestine of marron fed FPBM. Marron fed FPBM showed up-regulated expression of IL-8, IL-10, and IL-17F genes in the distal intestine. Significantly ( P < 0.05) increased lysozyme and phagocytic activity, and higher survival was found in marron fed FPBM following a bacterial challenge with Vibrio mimicus . Therefore, it is concluded that FPBM is beneficial to marron in terms of microbial community, immune-related cytokines and disease resistance against V. mimicus.