Nanobubble Mediated Gene Delivery in Conjunction With a Hand-Held Ultrasound Scanner | Zendy

Hiroshi Kida | Zendy; Koyo Nishimura | Zendy; Koki Ogawa | Zendy; Akiko Watanabe | Zendy; Loreto B. Feril | Zendy; Yutaka Irie | Zendy; Hitomi Endo | Zendy; Shigeru Kawakami | Zendy; Katsuro Tachibana | Zendy

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Nanobubble Mediated Gene Delivery in Conjunction With a Hand-Held Ultrasound Scanner

Author(s) -

Hiroshi Kida,

Koyo Nishimura,

Koki Ogawa,

Akiko Watanabe,

Loreto B. Feril,

Yutaka Irie,

Hitomi Endo,

Shigeru Kawakami,

Katsuro Tachibana

Publication year - 2020

Publication title -

frontiers in pharmacology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.384

H-Index - 86

ISSN - 1663-9812

DOI - 10.3389/fphar.2020.00363

Subject(s) - transfection , genetic enhancement , in vitro , gene transfer , gene delivery , albumin , gene , medicine , microbiology and biotechnology , biomedical engineering , cancer research , computational biology , chemistry , biology , biochemistry

Recent research has revealed that nanobubbles (NBs) can be an effective tool for gene transfection in conjunction with therapeutic ultrasound (US). However, an approach to apply commercially available hand-held diagnostic US scanners for this purpose has not been evaluated as of now. In the present study, we first compared in vitro , the efficiency of gene transfer (pCMV-Luciferase) with lipid-based and albumin-based NBs irradiated by therapeutic US (1MHz, 5.0 W/cm 2 ) in oral squamous carcinoma cell line HSC-2. Secondly, we similarly examined if gene transfer in mice is possible using a clinical hand-held US scanner (2.3MHz, MI 1.0). Results showed that lipid-based NBs induced more gene transfection compared to albumin-based NBs, in vitro . Furthermore, significant gene transfer was also obtained in mice liver with lipid-based NBs. Sub-micro sized bubbles proved to be a powerful gene transfer reagent in combination with conventional hand-held ultrasonic diagnostic device.

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