English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

Previously, we showed vascular endothelial overexpression of human-CYP2J2 enhances coronary reactive hyperemia in Tie2-CYP2J2 Tr mice, and eNOS −/−  mice had overexpression of CYP2J-epoxygenase with adenosine A 2A  receptor-induced enhance relaxation, but we did not see the response in CYP2J-epoxygenase knockout mice. Therefore, we hypothesized that  Cyp2j5 -gene deletion affects acetylcholine- and 5'-N-ethylcarboxamidoadenosine (NECA) (adenosine)-induced relaxation and their response is partially inhibited by angiotensin-II (Ang-II) in mice. Acetylcholine (Ach)-induced response was tested with  N -(methylsulfonyl)-2-(2-propynyloxy)-benzenehexanamide (MS-PPOH, CYP-epoxygenase inhibitor; 10 −5 M) and Ang-II (10 −6 M). In  Cyp2j5 −/−   mice, ACh-induced relaxation was different from C57Bl/6 mice, at 10 −5  M (76.1 ± 3.3  vs.  58.3 ± 5.2,  P  < 0.05). However, ACh-induced relaxation was not blocked by MS-PPOH in  Cyp2j5 −/−  : 58.5 ± 5.0%,  P  > 0.05, but blocked in C57Bl/6: 52.3 ± 7.5%,  P  < 0.05, and Ang-II reduces ACh-induced relaxation in both  Cyp2j5 −/−   and C57Bl/6 mice (38.8 ± 3.9% and 45.9 ± 7.8,  P < 0.05). In addition, NECA-induced response was tested with Ang-II. In  Cyp2j5 −/−   mice, NECA-induced response was not different from C57Bl/6 mice at 10 −5 M (23.1 ± 2.1  vs.  21.1 ± 3.8,  P  > 0.05). However, NECA-induced response was reduced by Ang-II in both  Cyp2j5 −/−   and C57Bl/6 mice (−10.8 ± 2.3% and 3.2 ± 2.7,  P <  0.05). Data suggest that ACh-induced relaxation in  Cyp2j5 −/−   mice depends on nitric oxide (NO) but not CYP-epoxygenases, and the NECA-induced different response in male  vs.  female  Cyp2j5 −/−   mice when Ang-II treated.

Cyp2j5-Gene Deletion Affects on Acetylcholine and Adenosine-Induced Relaxation in Mice: Role of Angiotensin-II and CYP-Epoxygenase Inhibitor