Open AccessMetabolomics Analysis of Hippocampus and Cortex in a Rat Model of Traumatic Brain Injury in the Subacute Phase
Author(s) -
Fei Zheng,
Yunfei Zhou,
Pengfei Li,
En Hu,
Teng Li,
Tao Tang,
Jiayou Luo,
Wei Zhang,
Changsong Ding,
Yang Wang
Publication year - 2020
Publication title -
frontiers in neuroscience
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.499
H-Index - 102
eISSN - 1662-4548
pISSN - 1662-453X
DOI - 10.3389/fnins.2020.00876
Subject(s) - traumatic brain injury , hypotaurine , metabolomics , hippocampal formation , neuroscience , medicine , hippocampus , taurine , bioinformatics , biology , biochemistry , psychiatry , amino acid
Traumatic brain injury (TBI) is a complex and serious disease as its multifaceted pathophysiological mechanisms remain vague. The molecular changes of hippocampal and cortical dysfunction in the process of TBI are poorly understood, especially their chronic effects on metabolic profiles. Here we utilize metabolomics-based liquid chromatography coupled with tandem mass spectrometry coupled with bioinformatics method to assess the perturbation of brain metabolism in rat hippocampus and cortex on day 7. The results revealed a signature panel which consisted of 13 identified metabolites to facilitate targeted interventions for subacute TBI discrimination. Purine metabolism change in cortical tissue and taurine and hypotaurine metabolism change in hippocampal tissue were detected. Furthermore, the associations between the metabolite markers and the perturbed pathways were analyzed based on databases: 64 enzyme and one pathway were evolved in TBI. The findings represented significant profiling changes and provided unique metabolite–protein information in a rat model of TBI following the subacute phase. This study may inspire scientists and doctors to further their studies and provide potential therapy targets for clinical interventions.