Open AccessNutrient status, hydrogen peroxide content and peroxidase activity of arbuscular mycorrhizal plants of Melilotus albus grown in diesel-contaminated substrate
Author(s) -
Herminia Alejandra Hernández-Ortega,
Ronald FerreraCerrato,
Humberto A. López-Delgado,
Juan Carlos Sánchez-Rangel,
Alejandro Alarcón
Publication year - 2021
Publication title -
scientia fungorum
Language(s) - English
Resource type - Journals
ISSN - 2594-1321
DOI - 10.33885/sf.2021.51.1298
Subject(s) - diesel fuel , contamination , nutrient , agronomy , biology , chemistry , horticulture , ecology , organic chemistry
Background: Petroleum hydrocarbons affect plant growth, but little is known about physiological responses of mycorrhizal plants facing diesel contamination.
Objective: To evaluate the effects of arbuscular mycorrhizal fungi (AMF) on the nutritional status, peroxidase activity (POX), and hydrogen peroxide content (H2O2) in leaves of Melilotus albus planted under diesel-contaminated sand (7500 mg kg-1).
Methods: A 2x2 factorial experiment was set in a completely randomized design, under greenhouse conditions for 35 days. Seedlings were pre-inoculated with AMF and transplanted to sand with or without diesel, including non-AMF plants.
Results and conclusions: Diesel contamination impaired plant growth; AMF plants had similar growth than non-AMF plants at diesel-contamination, but low nutrient content. Protein content decreased due to diesel in non-AMF plants, but this content was low in AMF plants regardless diesel contamination. Diesel increased POX; whereas AMF plants with or without diesel had higher POX than non-AMF plants. The H2O2 content in AMF plants with or without diesel was low than non-AMF plants. Diesel contamination diminished AMF-colonization, but AMF dissipate more diesel hydrocarbons (>40%). Overall, AMF alleviated the toxic effects of diesel on plants.