Open AccessDIAGNOSTIC VALUE OF EXOSOMAL MIRNAS FOR COLORECTAL CANCER
Author(s) -
Roman Samsonov,
Mikhail Tarasov,
Vladimir Burdakov,
Tatiana Shtam,
А. М. Гуляев,
O. A. Tkachenko,
Е. Г. Рыбаков,
М. В. Филатов,
Achim Aigner,
Anastasia Malek
Publication year - 2018
Publication title -
koloproktologiâ
Language(s) - English
Resource type - Journals
eISSN - 2686-7303
pISSN - 2073-7556
DOI - 10.33878/2073-7556-2018-0-2-25-31
Subject(s) - microrna , colorectal cancer , microvesicles , exosome , medicine , oncology , cancer , flow cytometry , biology , immunology , gene , genetics
OBJECTIVE. Assessment of diagnostic significance of exosomal microRNAs analysis in colorectal cancer (CRC). MATERIALS AND METHODS. Plasma samples from 100 patients with colon tumors and 20 healthy donors. Exosomes were isolated by differential ultracentrif ugation the analysis was performed by dynamic light scattering (DLS), cryo-EM, flow cytometry. Quantitative analysis of exosomal microRNAs was performed by RT-PCR. To evaluate the results obtained, the Kraskel-Wallis statistical test and ROC analysis were used. RESULTS. It is shown that CRC causes characteristic changes in the concentration of a number of exosomal microRNAs. Analysis of «reciprocal miRNAs pairs» was proposed as algorithmfor personalized diagnostic of CRC. The optimal parameters of diagnostic values were obtained for miRNA pair«miR-223 / miR-181a» (sensitivity =0.93, specificity =0.88). CONCLUSION. The analysis of exosomal microRNAs presents a promising method for early diagnostics / screening of colorectal cancer.