Open AccessEFFECT OF PHYSIOLOGICAL PARAMETERS ON MASS PRODUCTION OF TRICHODERMA SPECIES
Author(s) -
Muhammad Usman Ghazanfar,
Mubashar Raza,
Waqas Raza
Publication year - 2018
Publication title -
pakistan journal of phytopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.151
H-Index - 3
eISSN - 2305-0284
pISSN - 1019-763X
DOI - 10.33866/phytopathol.030.01.0447
Subject(s) - trichoderma harzianum , trichoderma , biology , nitrate , sucrose , ammonium , biomass (ecology) , nitrite , botany , potassium nitrate , rhizosphere , ammonium nitrate , biological pest control , horticulture , nitrogen , food science , chemistry , potassium , agronomy , bacteria , ecology , genetics , organic chemistry
Mycophagous fungi show antagonistic potential against plant pathogenic fungi and suggested as a promising biocontrol agent that influences by nutritional and environmental parameters. The present research was conducted with the aim to determine optimal environmental and nutritional parameters range for biomass production of Trichoderma. The two species of Trichoderma isolated from rhizosphere of citrus, wheat and tomato of different localities of district Sargodha, Punjab, Pakistan and later on effect of temperature, carbon, nitrogen and pH were investigated on three strain of T. harzianum HM, HK, HC and one strain of T. asperellum TH under in vitro conditions. All strains produced statistically sufficient mass production at all temperatures but 25 °C as well as 30 °C were found ideal for mass production of Trichoderma. A substantial difference in mass production of Trichoderma isolates was recorded at different pH levels includes 4.0, 5.0, 6.0, 7.0 and 8.0. The pH range from 5.0 to 7.0 found to be optimum for all species of Trichoderma. All fungal species of Tricoderma produced extensive mass production on growth media supplemented with carbon and nitrogen sources such as mannose, glactose, sucrose, lactose and sodium nitrite (NaNO3), potassium nitrate (KNO3), ammonium nitrate (NH4NO3) as well as ammonium nitrite (NH4NO3) respectively. The optimal mass production was recorded on carbon supplemented growth medium as compared to nitrogen supplemented growth medium. Therefore, T. harzianum HK produced maximum biomass production on among all isolates of Trichoderma.