Open AccessDevelopmentofin-vitroSensitivityTestingforPathogenicBacteria
Author(s) -
Fouad Houssein Kamel,
Chiman Hameed Saeed,
Ashti M.Amin,
Saleem S. Qader
Publication year - 2018
Publication title -
mağallaẗ al-qādisiyyaẗ li-l-ʿulūm al-zirāʿiyyaẗ/al-qadisiyah journal for agriculture sciences
Language(s) - Uncategorized
Resource type - Journals
eISSN - 2618-1479
pISSN - 2077-5822
DOI - 10.33794/qjas.vol8.iss2.54
Subject(s) - ampicillin , microbiology and biotechnology , chemistry , biology , antibiotics
Anewmethod developed for in vitrosusceptibility test in medicall aboratoriesconsistofmicrotubesorglovescontainingdehydratedtrypticsoyabroth,5%glucose,0.1%bromothymolblueandonetypeofantibiotics(ampicillin,tetracyclineandchloramphenicol)withcriticalconcentrationMIC(minimuminhibitoryconcentration)forsusceptibility.Standardqualitycontrolstrainsofbacterial(Escherichiacoli,StaphylococcusaureusandPseudomonasaeruginosa)suspensionwereadjustedto0.5McFarlandturbiditystandard(1×106cell/mL)wereusedininoculationthemediaandincubatedtwohoursat37°C.TheMICofampicillinagainstE.coli,S.aureus,andP.aeruginosawere4,32,and256µg/mLofthemediaforthebacteriarespectively,whiletheMICoftetracyclineagainstbacteriawere512,512and32µg/mLrespectively,theMICofchloramphenicolwere512,32and512µg/mL,respectively.Where,theresistantbacteriatotheantibioticscouldgrowandfermentglucosesugarproducingacolorchangeofthemediafrombluetoyellow,whilethesensitivebacteriadonotgroworshownochangeincolor.Ourstudyresultcomparedwithcommonusedantibioticdiskmethodobtainingsimilarresults.Thisdevelopedmethodcharacterizedbyfast(onlytwohours)andlesscostincomparisontoconventionaltechnique.Thenewmicrotubestripishighlystable(morethanoneyear)withmoresensitiveindetectionofvariablepathogenicbacteriaincludingstandardbacteriastrainscomparedwithconventionaltechnique.