Open AccessIdentification and Confirmation of Chironomus circumdatus Kieffer, 1916 (Diptera: Chironomidae) Using DNA Barcoding
Author(s) -
Deepak Rawal,
Verma Himani,
Prajapat Gyata
Publication year - 2021
Publication title -
international journal of zoological investigations
Language(s) - English
Resource type - Journals
ISSN - 2454-3055
DOI - 10.33745/ijzi.2021.v07i01.025
Subject(s) - dna barcoding , chironomidae , chironomus , biology , mitochondrial dna , cytochrome c oxidase subunit i , taxonomy (biology) , molecular taxonomy , larva , dna sequencing , zoology , phylogenetics , evolutionary biology , gene , ecology , genetics
Present study was performed to identify and confirm collected larval Chironomid up to species level. Toassess molecular taxonomy and phylogeny, DNA barcoding was done using Sanger dideoxy sequencing ofmitochondrial cytochrome c oxidase subunit I (COI) gene of larval sample. Bioinformatics analysis was done byusing NCBI’s BLAST software. By evidence of DNA barcoding, it was confirmed that present larval chironomidspecies was Chironomus circumdatus Kieffer, 1916.