Open AccessIn Vitro Assessment of the Antimicrobial Efficacy of Chitosan from Scylla serrata Against the Human Pathogens
Author(s) -
B Sangeetha,
V. Indra,
Abdul Rahim M,
E Venkadachalam
Publication year - 2021
Publication title -
international journal of zoological investigations
Language(s) - English
Resource type - Journals
ISSN - 2454-3055
DOI - 10.33745/ijzi.2021.v07i01.016
Subject(s) - scylla serrata , chitosan , antibacterial activity , antimicrobial , bacillus cereus , microbiology and biotechnology , chitin , biology , food science , chemistry , bacteria , biochemistry , fishery , genetics
Chitosan, a polysaccharide derivative of chitin forms the structural components in the exoskeletons ofcrustacean animals and is also found in the cell walls of fungi. Chitosan is produced primarily from the crab shellwaste through chemical means. The chemical structure of chitosan has been identified and characterized by FT-IRand XRD. In the current study, the antimicrobial activity of chemically deacetylated chitosan extracts of mud crabs(Scylla serrata) was evaluated against five human pathogenic isolates viz. Staphylococcus aureus, Salmonella typhi,Klebsiella pneumonia, Bacillus cereus and Pseudomonas aeruginosa using agar disc diffusion method. These wereperformed to determine the crystallinity and functional properties of chitosan. The results showed that theantibacterial effect increased with increasing chitosan concentration. This study showed that chitosan isolated fromcrab shell has potential antibacterial activity therefore it can be utilized in the food and pharmaceutical industries,and that antibacterial activity may be due to functional groups present in the crab shell.