Open AccessProlactin induced cyto-architectural changes in the corpuscles of Stannius of stinging catfish, Heteropneustes fossilis acclimated to different calcium media
Author(s) -
Srivastav Susmita,
Devesh Mishra,
Abhishek Kumar,
Srivastav Sunil K,
Norihiro Suzuki,
Srivastav Ajai K
Publication year - 2021
Publication title -
international journal of biological and environmental investigations
Language(s) - English
Resource type - Journals
ISSN - 2583-1690
DOI - 10.33745/ijbei.2021.v01i01.001
Subject(s) - heteropneustes fossilis , calcium , medicine , endocrinology , prolactin , chemistry , freshwater fish , fish <actinopterygii> , catfish , biology , hormone , fishery
Adult fish Heteropneustes fossilis were divided into 4 groups –(i) Group A: kept in artificial freshwaterand daily injected intraperitoneally with vehicle; (ii) Group B: kept in artificial freshwater and were dailyinjected intraperitoneally with 0.1 mg/100 g body wt of oProlactin; (iii) Group C: maintained in calcium-deficientfreshwater and daily injected intraperitoneally with vehicle; (iv) Group D: kept in calcium-deficient freshwaterand daily injected intraperitoneally with 0.1 mg/100 g body wt of oProlactin. Blood samples were taken 2 h afterthe last injection on 1, 3, 5, 10 and 15 days of the treatment. Plasma calcium levels were analyzed. The corpusclesof Stannius (CS) were fixed for histological studies.Artificial freshwater: The plasma calcium levels of vehicle-injected specimens (group A) remained unalteredthroughout the experiment. Following prolactin treatment (group B) the plasma calcium levels progressivelyincreased from day 3 to day 5. The values became normocalcemic at day 10 and day 15. After day 5 following prolactin administration (group B), the nuclear volume of AF-positive cells increasedand the cells were seen degranulated. After day 10, there was an increased dilatation of sinusoids and the nuclearvolume of AF-positive cells showed further increase. On day 15, these changes were exaggerated. The AFnegative cells of the corpuscles of Stannius of prolactin-treated fish (group B) showed no change in theirhistological structure and nuclear volume.Calcium-deficient freshwater: The plasma calcium level decreased in vehicle-injected fish (group C) from day 1 today 3 (as compared to level of the fish kept in artificial freshwater). Thereafter, the level increased from day 5resulting in hypercalcemia at day 10 and day 15. In prolactin treated fish (group D) the plasma calcium levelindicated progressive increase from day 5 to day 15. In the vehicle-injected fish (group C) the AF-positive cells of corpuscles of Stannius showed accumulati