Open AccessGene-Based Markers in Marker-Assisted Selection to Screen Tomato Genotypes Resistant to Fusarium Wilt, Late Blight, Verticillium Wilt, Leaf Mold, Bacterial Spot and Bacterial Speck
Author(s) -
Heba A. Mahfouze,
Sherin A. Mahfouze
Publication year - 2021
Publication title -
international journal of phytopathology
Language(s) - English
Resource type - Journals
eISSN - 2313-1241
pISSN - 2312-9344
DOI - 10.33687/phytopath.010.02.3637
Subject(s) - verticillium wilt , biology , verticillium dahliae , fusarium wilt , bacterial wilt , verticillium , marker assisted selection , pathosystem , fusarium oxysporum , phytophthora infestans , blight , plant disease resistance , cladosporium , xanthomonas , horticulture , genotype , botany , gene , genetics , aspergillus , inoculation
The tomato crop is exposed to serious losses due to infection with several diseases and pests, which threaten tomato production in Egypt and worldwide. Therefore, selecting the tomato germplasm resistant to a specific pathogen by molecular markers closely linked to resistance loci is a desirable goal of this study. In this work, seven co-dominant markers targeting six resistance genes (I-1, Ve, Ph3, Cf-9/Cf-4, Rx4, and Pto) for six main diseases [ fusarium wilt (Fusarium oxysporum f. sp. lycopersici), verticillium wilt (Verticillium dahliae and V. alboatrum), late blight (Phytophthora infestans), leaf mold (Cladosporium fulvum), bacterial spot (Xanthomonas campestris pv. vesicatoria) and bacterial speck (Pseudomonas syringae pv. tomato)], respectively were determined. Theses molecular markers differentiated among 19 tomato genotypes resistant (homozygote/heterozygote) and susceptible (homozygote) to the pathogens. Therefore, this study supplied us with novel tomato lines with resistance to multiple diseases, and their pyramiding inside domesticated tomato cultivars are suggested to apply in the tomato breeding programs of resistance against fungal and bacterial diseases.