Survey and Genetic Diversity of Grapevine Leafroll Associated Virus 2 in Algeria | Zendy

Arezki Lehad | Zendy; Ilhem Selmi | Zendy; Meriem Louanchi | Zendy; Mouni Aitouada | Zendy; Naima Mahfoudhi | Zendy

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Survey and Genetic Diversity of Grapevine Leafroll Associated Virus 2 in Algeria

Author(s) -

Arezki Lehad,

Ilhem Selmi,

Meriem Louanchi,

Mouni Aitouada,

Naima Mahfoudhi

Publication year - 2015

Publication title -

international journal of phytopathology

Language(s) - English

Resource type - Journals

eISSN - 2313-1241

pISSN - 2312-9344

DOI - 10.33687/phytopath.004.01.1074

Subject(s) - genbank , genetic diversity , phylogenetic tree , biology , genetics , population , gene , polymerase chain reaction , synonymous substitution , virology , genome , demography , sociology , codon usage bias

Vineyards in western and center regions of Algeria were surveyed for the Grapevine leafroll-associated virus 2 (GLRaV-2). Analyses by DAS-ELISA and Reverse Transcription Polymerase Chain Reaction (RT-PCR) reveal 15, 8% prevalence. The genetic diversity of the GLRaV-2 population was studied by phylogenetic analyses of the HSP70h gene region of seven samples sequenced in this study and other sequences downloaded from GenBank. Results reveal segregation of the GLRav-2 population into six distinct groups. An estimation of the ratio of non-synonymous substitutions per non-synonymous site to synonymous substitutions per synonymous site indicated that HSP70h gene evolve under positive selection. Similarity plot constructed with representative sequence from each group confirmed previous results. All Algerian isolates belong to group PN. As far as we know, this is the first characterization of GLRaV-2 isolates from Algeria.

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