Open AccessComparison of methods of surgical transplantation of mice embryos into pseudopregnant female recipients
Author(s) -
Е. С. Савченко,
Н. С. Огнева,
С В Максименко,
О. Б. Жукова
Publication year - 2021
Publication title -
biomedicina
Language(s) - English
Resource type - Journals
eISSN - 2713-0428
pISSN - 2074-5982
DOI - 10.33647/2713-0428-17-3e-80-88
Subject(s) - oviduct , infundibulum , transplantation , embryo , embryo transfer , andrology , pregnancy rate , pregnancy , biology , medicine , gynecology , surgery , anatomy , genetics , microbiology and biotechnology
There are a lot of factors that are able to influence the result of embryo transfer to pseudopregnant females, including the transplantation procedure itself. To determine the possible role of the surgical transplantation method on the embryo transfer result, we analyzed the data of transplantation of 6715 microinjected embryos into the oviduct of 471 pseudopregnant female by three ways — into the infundibulum, into the ampullae by the oviduct muscular wall puncture or incision. A significant increase in the pregnancy rate of recipients, as well as the birth rate in total recipients in oviduct puncture/incision groups, has been shown, however, the birth rate in pregnant recipients does not differ between all experimental groups. Survival of pups is also significantly higher in oviduct puncture/incision groups.