Comparison of exosome isolation methods for biomedical research | Zendy

Natalia Ponomareva | Zendy; Anastasiya Kostyusheva | Zendy; Sergey Brezgin | Zendy; Smirnov Vv | Zendy; V. I. Gegechkory | Zendy; Dmitry Kostyushev | Zendy; Vladimir Chulanov | Zendy

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Comparison of exosome isolation methods for biomedical research

Author(s) -

Natalia Ponomareva,

Anastasiya Kostyusheva,

Sergey Brezgin,

Smirnov Vv,

V. I. Gegechkory,

Dmitry Kostyushev,

Vladimir Chulanov

Publication year - 2021

Publication title -

journal biomed

Language(s) - English

Resource type - Journals

eISSN - 2713-0428

pISSN - 2074-5982

DOI - 10.33647/2713-0428-17-3e-76-79

Subject(s) - microvesicles , exosome , isolation (microbiology) , extracellular vesicles , ultracentrifuge , vesicle , microbiology and biotechnology , drug delivery , chemistry , extracellular vesicle , cell , membrane , computational biology , chromatography , biology , biochemistry , bioinformatics , microrna , organic chemistry , gene

Exosomes are nanoscale membrane vesicles secreted by almost all kinds of cells and stably existing in the body. Due to their unique properties, exosomes can be used in biomedical research as regenerative preparations or drug delivery vehicles. In this study, an anion exchange (AIEX) chromatography-based protocol for isolation of extracellular vesicles from a cell-conditioned medium was developed. The exosome isolation method based on AIEX chromatography overperforms the canonical ultracentrifugation-based isolation method in terms of its capability of large-volume processing.

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