Assessment of nuclear material elimination by different methods of dermis decellularization

We carry out a comparative assessment of the degree of nuclear material disintegration and elimination in the samples of decellularized porcine dermis after using chemical, detergent and enzymatic decellularization methods. Decellularized dermis materials are promising materials as wound dressings. The chemical method (Protocol No. 1) was performed using NaOH and H2 O2 solutions; the detergent method (Protocol No. 2) involved the solutions of Triton X-100 and sodium deoxycholate in combination with Na2 -EDTA; the enzymatic method (Protocol No. 3) was based on the solutions of trypsin Versene and porcine pan creatic DNase. Subsequently, we analyzed the DNA amount in decellularized and native dermis (control group) samples. The results of this analysis showed positive results in all three protocols. It was found that the detergent method have advantages over other methods of producing decellularized dermis matrices.