Open AccessMethodical aspects of lipopolysaccharides obtaining and characterization from Escherichia coli cells
Author(s) -
R. A. Ageldinov,
А. И. Левашова,
В. С. Кохан,
М. С. Нестеров
Publication year - 2021
Publication title -
biomedicina
Language(s) - English
Resource type - Journals
eISSN - 2713-0428
pISSN - 2074-5982
DOI - 10.33647/2713-0428-17-3e-14-16
Subject(s) - limulus amebocyte lysate , escherichia coli , chromatography , lipopolysaccharide , lysis , chemistry , extraction (chemistry) , high performance liquid chromatography , limulus , microbiology and biotechnology , biochemistry , biology , paleontology , gene , endocrinology
In this study, a procedure was applied to purify lipopolysaccharides from Escherichia coli based on a hot phenolic extraction protocol. The purity of the extracted lipopolysaccharides was assessed by HPLC-UV. Pyrogenic activity was determined using the Limulus Amebocyte Lysate test and used to monitor the functionality of the purified lipopolysaccharides. HPLC analysis showed a high degree of purity comparable to commercial lipopolysaccharide. Pyrogenic activity confirmed the functional activity of purified lipopolysaccharides. The presented protocol can be used to isolate lipopolysaccharides with high purity and functional activity.