Open AccessInvestigation of the adjuvant properties of nanoparticles from a birch bark extract used for immunization of mini pigs of the Svetlogorsk population
Author(s) -
А. Г. Берзина,
Н. В. Станкова,
Alexander Kaplun,
A. A. Burkova,
N. B. Gamaleya
Publication year - 2021
Publication title -
biomedicina
Language(s) - English
Resource type - Journals
eISSN - 2713-0428
pISSN - 2074-5982
DOI - 10.33647/2713-0428-17-3e-133-138
Subject(s) - immunogen , adjuvant , immunization , bark (sound) , titer , population , aqueous suspension , antibody titer , antigen , chemistry , immune system , antibody , immunology , medicine , biology , aqueous solution , monoclonal antibody , ecology , environmental health
The adjuvant properties of nanoparticles derived from a dry birch bark extract were studied. Immunization experiments were carried out on mini pigs of the Svetlogorsk population, a human biomodel. Animals received two subcutaneous injections of the antigen at a dose of 0.1 mg/kg with an interval of 14 days. The addition of an aqueous suspension of nanoparticles to the immunogen samples up to a final concentration of 0.5 mg/ml led to an increase in the immune response. No side effects were observed. The antibody titers in blood sera on the 60th and 90th days from the start of immunization were comparable to those obtained under similar conditions using Freund’s adjuvant (1:4000).