Open AccessPreparation, Characterization and Preliminary <i>in vivo</i> Testing of Liposomated Antiidiotypic Antibodies Against Morphine Derivatives
Author(s) -
V. N. Karkischenko,
А. Г. Берзина,
Т. А. Климова,
N. B. Gamaleya,
R. A. Ageldinov,
Alexey Kuznetsov,
I. S. Korsun,
М. С. Нестеров,
L.I. Ulyanova
Publication year - 2021
Publication title -
biomedicina
Language(s) - English
Resource type - Journals
eISSN - 2713-0428
pISSN - 2074-5982
DOI - 10.33647/2074-5982-17-4-8-17
Subject(s) - liposome , in vivo , adjuvant , zeta potential , pharmacology , antibody , chemistry , medicine , materials science , nanotechnology , biochemistry , immunology , nanoparticle , biology , microbiology and biotechnology
Articial membrane structures containing medicinal substances are highly promising for the development of new drugs. Liposomal preparations are actively used in medical practice due to their high efcacy and relatively low toxicity. Our aim was to encapsulate anti-idiotypic antibodies into a liposomal composition with the purpose of improving their immunogenic properties. Following the preparation of a liposomal composition by the dehydration/rehydration method using ultrasonic treatment, the size, zeta potential, and loading efciency of liposomes were investigated. Preliminary in vivo studies were conducted to evaluate the adjuvant properties of liposomes of varying size. Loaded liposomes of the smallest diameter (about 110 nm) showed the potential of enhancing the immune response similar to that obtained using Freund’s adjuvant. These results justify further research into the properties of liposomes loaded with antibodies.