Open AccessAn Experimental Model of Direct Acute Lung Injury in Rats Caused by Intratracheal Administration of Lipopolysaccharide from Salmonella enterica
Author(s) -
В. А. Пугач,
М. А. Тюнин,
Н. С. Ильинский,
Е. В. Левчук,
Е. И. Строкина,
А. А. Ельцов
Publication year - 2021
Publication title -
biomedicina
Language(s) - English
Resource type - Journals
eISSN - 2713-0428
pISSN - 2074-5982
DOI - 10.33647/2074-5982-17-3-84-89
Subject(s) - lipopolysaccharide , salmonella enterica , medicine , lung , ards , salmonella , acute respiratory distress , pulmonary edema , edema , pharmacology , immunology , bacteria , biology , genetics
An experimental model of direct acute lung injury was developed by intratracheal administration of lipopolysaccharide from Salmonella enterica (LD50 = 20 mg/kg). The dynamics of animal lethality, body weight, temperature and the severity of pathomorphological changes in the lung tissue were analyzed. It was found that the developed model is accompanied by a progressive decrease in body weight by 15%, persistent hypothermic reaction, pronounced edema and inflammatory reaction in the lung tissue within 4 days following lipopolysaccharide administration. The simplicity and reproducibility of the developed experimental model make it useful for preclinical research aimed at selection of candidate drugs for the prevention and treatment of acute respiratory distress syndrome.