Open AccessApplication of Biomolecular Methods for Estimating Cytokine and Sirtuin Expression in Biosimulation of Ultimate Physical Load of Athletes
Author(s) -
V. N. Karkischenko,
Н. В. Петрова,
V. V. Slobodenyuk,
N. A. Laryushina
Publication year - 2021
Publication title -
biomedicina
Language(s) - English
Resource type - Journals
eISSN - 2713-0428
pISSN - 2074-5982
DOI - 10.33647/2074-5982-17-3-10-16
Subject(s) - sirtuin , cytokine , sirtuin 1 , athletes , activator (genetics) , rothschild , biology , gene , medicine , computational biology , bioinformatics , immunology , genetics , physical therapy , downregulation and upregulation , acetylation , history , archaeology
Mini-pigs are an adequate biomodel for characterizing the processes of working capacity and endurance, as well as for conducting investigations in the field of sports medicine. For this kind of research, we propose gene targets from the families of cytokine and sirtuin proteins: IL-6, HMGB1, TNF (genes responsible for the synthesis of proteins belonging to the cytokine group) and SIRT (transferase from the family of sirtuin proteins). The SIRT 1 gene presents particular interest as an activator of mitochondrial activity during exercise. Real-time PCR systems were created, allowing assessment of the effect of various drugs on laboratory mini-pigs in preclinical studies.