Open AccessOptimizing the Hormonal Mice Treatment Protocol to Induce Superovulation
Author(s) -
Е. С. Савченко,
Н. С. Огнева,
С В Максименко,
М. М. Скрипкина,
Н. В. Петрова
Publication year - 2020
Publication title -
biomedicina
Language(s) - English
Resource type - Journals
eISSN - 2713-0428
pISSN - 2074-5982
DOI - 10.33647/2074-5982-16-3-48-53
Subject(s) - human chorionic gonadotropin , hormone , embryo , gonadotropin , medicine , andrology , endocrinology , physiology , biology , microbiology and biotechnology
Mice treatment with pregnant mare gonadotropin serum (PMGS) in combination with human chorionic gonadotropin (hCG) is considered to be the “golden standard” of the induced superovulation procedure. The effect of administering the same doses may differ depending on the manufacturer of the preparation. According to our results, the use of PMGS produced by Synchrostim 500 (Ceva Sante Animale, France) and Sergon (Bioveta, Czech Republic) at a hormone dose of 5ME allows ~25 embryos to be obtained from one female. At the same time, the working dose of Follimagum (Mosagrogen, Russia) was not determined. The result comparable with that of Synchrostim 500 and Sergon was achieved using a composite drug Menopur (Ferring, Germany). The study of the inuence of mice age on the hormonal response showed 3–4 weeks to be the most productive age, when the maximal number of viable embryos was obtained under a minimal external invasion.