Open AccessLeptin Resistance in the Ovary of Obese Mice is Associated with Profound Changes in the Transcriptome of Cumulus Cells
Author(s) -
Karolina Wołodko,
Edyta Walewska,
Marek Adamowski,
Juan Castillo-Fernandez,
Gavin Kelsey,
António Galvão
Publication year - 2020
Publication title -
cellular physiology and biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.486
H-Index - 87
eISSN - 1421-9778
pISSN - 1015-8987
DOI - 10.33594/000000228
Subject(s) - transcriptome , leptin , medicine , biology , endocrinology , socs3 , leptin receptor , adipokine , lipotoxicity , microbiology and biotechnology , insulin resistance , obesity , stat3 , gene expression , signal transduction , gene , genetics
Obesity is associated with infertility, decreased ovarian performance and lipotoxicity. However, little is known about the aetiology of these reproductive impairments. Here, we hypothesise that the majority of changes in ovarian physiology in diet-induced obesity (DIO) are a consequence of transcriptional changes downstream of altered leptin signalling. Therefore, we investigated the extent to which leptin signalling is altered in the ovary upon obesity with particular emphasis on effects on cumulus cells (CCs), the intimate functional companions of the oocyte. Furthermore, we used the pharmacological hyperleptinemic (LEPT) mouse model to compare transcriptional profiles to DIO.