Open AccessAR1 quality assurance by ELISA
Author(s) -
Lyn Briggs,
J. M. Sprosen,
B.A. Tapper,
H. S. Easton
Publication year - 2007
Publication title -
grassland research and practice series
Language(s) - English
Resource type - Journals
eISSN - 2463-4751
pISSN - 0110-8581
DOI - 10.33584/rps.13.2006.3145
Subject(s) - contamination , endophyte , quality assurance , sample (material) , microbiology and biotechnology , toxicology , biology , business , botany , chemistry , marketing , ecology , chromatography , service (business)
Tests are required to monitor contamination of AR1-infected seed with ryegrass seed infected with the common toxic endophyte to ensure contamination is maintained below 5% for First Generation seed and below 2% for Breeders and Basic seed. To achieve this AR1 seed is tested for the presence of lolitrem toxins that are produced by the endophytic fungus in toxic seeds. This was done by HPLC analysis until 2006 when an ELISA method, more suited to processing large sample numbers, was successfully established as a replacement. The ELISA is specific for lolitrems and lolitriol, provides rapid sample turnaround and analytical costs have been reduced. It is anticipated that in the future the test will be transferred to a commercial testing laboratory and will be extended to monitor contamination of AR37 seed with common toxic ryegrass seed. Keywords: AR1 ryegrass, wild-type endophyte, lolitrem, ELISA, quality assurance