Open AccessAgrobacterium tumefaciens T-DNA mediated transformation of Epichloe festucae
Author(s) -
Atsushi Tanaka,
R.E. Wrenn,
Darin Takemoto,
Bari Scott
Publication year - 2007
Publication title -
grassland research and practice series
Language(s) - English
Resource type - Journals
eISSN - 2463-4751
pISSN - 0110-8581
DOI - 10.33584/rps.13.2006.3125
Subject(s) - biology , transformation (genetics) , agrobacterium tumefaciens , plasmid , genetics , agrobacterium , epichloë , mutagenesis , mutant , dna , symbiosis , gene , bacteria
Although much is known about the signals and mechanisms that lead to pathogenic interactions between plants and fungi comparatively little is known about fungal-plant mutualistic symbiotic interactions. The association between Epichloë festucae and perennial ryegrass is a powerful experimental system to understand the molecular basis for fungal-plant mutualistic interactions. We have adopted a forward genetics approach to dissect this symbiosis. Previously we described the use of plasmid mutagenesis to isolate a symbiotic mutant. We describe here the development of an Agrobacterium tumefaciens mediated T-DNA transformation system for E. festucae. Keywords: Epichloë festucae, Agrobacterium tumefaciens, TDNA, transformation