Open AccessRestriction-modification systems and DNA methylation profile of Pectobacterium carotovorum 2A
Author(s) -
Yulia V. Diubo,
A. E. Akhremchuk,
Л. Н. Валентович,
Yevgeny Nikolaichik
Publication year - 2021
Publication title -
žurnal belorusskogo gosudarstvennogo universiteta. bilologiâ/žurnal belorusskogo gosudarstvennogo universiteta. biologiâ
Language(s) - English
Resource type - Journals
eISSN - 2617-3964
pISSN - 2521-1722
DOI - 10.33581/2521-1722-2021-3-71-77
Subject(s) - pectobacterium carotovorum , pectobacterium , biology , dna methylation , genetics , nanopore sequencing , dna , genome , amplified fragment length polymorphism , dna sequencing , microbiology and biotechnology , gene , medicine , population , gene expression , environmental health , genetic diversity
The methylation profile of Pectobacterium carotovorum 2A genome was studied using the Oxford Nanopore sequencing technology. The specificity of the methylase subunits of the three restriction-modification systems of this strain was determined. Analysis of homologous systems showed the uniqueness of the type I restriction-modification system and the type IV restriction system specific to methylated DNA of this strain. The work confirms the applicability of Oxford Nanopore technology to the analysis of bacterial DNA modifications and is also the first example of such an analysis for Pectobacterium spp.