Open AccessStudy of the ascorbate-glutathione cycle in transgenic plants Nicotiana tabacum under abiotic stress conditions
Author(s) -
Kristina V. Pristupa,
Tatsiana A. Kukulianskaya,
E.A. Khramtsova
Publication year - 2021
Publication title -
žurnal belorusskogo gosudarstvennogo universiteta. bilologiâ/žurnal belorusskogo gosudarstvennogo universiteta. biologiâ
Language(s) - English
Resource type - Journals
eISSN - 2617-3964
pISSN - 2521-1722
DOI - 10.33581/2521-1722-2021-2-11-18
Subject(s) - nicotiana tabacum , glutathione reductase , ascorbic acid , glutathione , transgene , peroxidase , genetically modified crops , nicotiana , biochemistry , biology , antioxidant , glutathione peroxidase , chemistry , botany , enzyme , horticulture , gene , solanaceae
We conducted a comparative analysis of biochemical parameters for the ascorbate-glutathione cycle in non-transgenic and transgenic Nicotiana tabacum, plants cultivated in heavy metal polluted soils. Transgenic plants had in their genome a bacterial acdS-gene encoding the 1-aminocyclopropane-1-carboxylate deaminase (ACC-deaminase) enzyme. The introduction of elevated concentrations of copper, chromium, and lead ions into the soil promotes induction of the acdS-gene expression and an increase in ACC-deaminase activity in transgenic plants. It was shown the content of ascorbic acid (АА), glutathione, ascorbate peroxidase (АРХ) and glutathione peroxidase (GP) and glutathione reductase (GR) increased in plants under abiotic stress.