Open AccessProperties of expression of protein capside porcine circovirus type 2 in bacterial cells
Author(s) -
Anastasia D. Titova,
Kirill V. Kudzin,
В. А. Прокулевич
Publication year - 2021
Publication title -
žurnal belorusskogo gosudarstvennogo universiteta. bilologiâ/žurnal belorusskogo gosudarstvennogo universiteta. biologiâ
Language(s) - English
Resource type - Journals
eISSN - 2617-3964
pISSN - 2521-1722
DOI - 10.33581/2521-1722-2021-1-48-57
Subject(s) - porcine circovirus , capsid , open reading frame , gene , microbiology and biotechnology , expression vector , biology , circovirus , vector (molecular biology) , stop codon , gene expression , genetics , recombinant dna , virus , peptide sequence
To improve expression of the porcine circovirus type 2 (PCV2) capsid protein in E. coli cells, the corresponding gene was optimized and two variants of the open reading frame were constructed, which encoded the full-sized and shortened capsid proteins as part of the expression vector. Rare codons were replaced, and in the case of a shortened version of the gene, the region corresponding to the N-terminal domain of the protein was deleted. A comparison was made of the expression level of the studied proteins. It was established that the highest level of expression in bacterial cells is achieved by simultaneously optimizing the codons and removing the initial (N-terminal) 108 base pair (bp) portion of the gene, which contains the nuclear localization signal.