Open AccessAstaxanthin accumulation in Haematococcus pluvialis cells induced by nitrogen deficiency and high light intensity
Author(s) -
T.V. Samovich,
Р. Г. Гончарик,
Elizaveta I. Pechenkina,
Yauhen V. Viazau,
Н. В. Козел
Publication year - 2020
Publication title -
žurnal belorusskogo gosudarstvennogo universiteta. bilologiâ/žurnal belorusskogo gosudarstvennogo universiteta. biologiâ
Language(s) - English
Resource type - Journals
eISSN - 2617-3964
pISSN - 2521-1722
DOI - 10.33581/2521-1722-2020-3-37-45
Subject(s) - haematococcus pluvialis , astaxanthin , pluvialis , biochemistry , nitrogen deficiency , light intensity , biosynthesis , nitrogen , chemistry , carotenoid , food science , biology , enzyme , botany , organic chemistry , physics , optics
It was established that nitrogen deficiency initiated at the resting phase of Haematococcus pluvialis (H. pluvialis) is not an effective stress factor in combination with high light intensity to induce the accumulation of astaxanthin in algae cells. The amounts of astaxanthin in H. pluvialis cells in the absence of nitrogen were lower compared to those when complete Rudic medium was used, regardless of the light intensity. This fact indicates the need for a certain amount of nitrogen in the medium for the functioning of the astaxanthin synthesis system. In addition, the low yield of astaxanthin in the experimental variants cultivated on incomplete Rudic medium is associated with the insufficient amount of nitrogen for its biosynthesis and also with a decrease in the expression level of the PSY gene encoding a key enzyme of the carotenoid biosynthesis chain – phytoene synthase.