Open AccessThe influence of bacteria acdS-gene of Pseudomonas putida B-37 on Nicotiana tabacum transgenic plants under abiotic stress conditions
Author(s) -
Daria A. Rutkevich,
Katsiaryna S. Karaleva,
E.A. Khramtsova
Publication year - 2020
Publication title -
žurnal belorusskogo gosudarstvennogo universiteta. bilologiâ/žurnal belorusskogo gosudarstvennogo universiteta. biologiâ
Language(s) - English
Resource type - Journals
eISSN - 2617-3964
pISSN - 2521-1722
DOI - 10.33581/2521-1722-2020-1-39-46
Subject(s) - nicotiana tabacum , pseudomonas putida , genetically modified crops , biology , transgene , abiotic component , abiotic stress , polymerase chain reaction , gene , bacteria , microbiology and biotechnology , botany , biochemistry , genetics , paleontology
In the present work was carried out selection of transgenic plants of Nicotiana tabacum carrying acdS-gene of bacteria Pseudomonas putida B-37. Using polymerase chain reaction with specific primers, the presence of the target gene was proved. Transgenic plants selected on a selective medium were planted in the ground and subjected to abiotic stress caused by soil contamination with heavy metal salts (15 mg / kg for Cu2+ and 30 mg / kg for Cr6+ and Pb2+ ) and salinization of the soil (200 mmol/L NaCl). Reverse transcription polymerase chain reaction and real-time polymerase chain reaction were conducted and confirmed the transcriptional activity of the bacterial acdS-gene in transgenic plant cells at a level with the reference gene Ef-1a. Determination of activity of ACC-deaminase, the product of expression of the acdS-gene, confirmed the formation of the active enzyme in the leaf tissues of transgenic tobacco plants. The beneficial effect of the acdS-gene of the bacteria P. putida B-37 on transgenic N. tabacum plants under abiotic stress has been proven.