Open AccessExpression analysis of acdS-gene of Pseudomonas putida B-37 in transgenic plants Nicotiana tabacum
Author(s) -
Alesia A. Melnikava,
Alena A. Khramtsova,
Katsiaryna S. Karaleva,
Daria A. Rutkevich,
Tatsiana A. Kukulianskaya
Publication year - 2019
Publication title -
žurnal belorusskogo gosudarstvennogo universiteta. bilologiâ/žurnal belorusskogo gosudarstvennogo universiteta. biologiâ
Language(s) - English
Resource type - Journals
eISSN - 2617-3964
pISSN - 2521-1722
DOI - 10.33581/2521-1722-2019-1-45-53
Subject(s) - nicotiana tabacum , pseudomonas putida , biology , transformation (genetics) , gene , genetically modified crops , heterologous expression , transgene , gene expression , agrobacterium tumefaciens , microbiology and biotechnology , plasmid , genetics , recombinant dna
In current work was realized the transfer of recombinant plasmid pBI121acdS, carring P. putida B-37 bacterial acdSgene to the A. tumefaciens AGL0 cells. Transgenic plants of N. tabacum were created by agrobacterial transformation. Integration of P. putida B-37 bacterial acdS-gene to transgenic plants of N. tabacum was verified by PCR analysis, using specific primers to present gene. Presence of target acdS-gene in transgenic plants genome was proved by RT-PCR analysis. With help of Real-time PCR was shown the difference between reference gene and target P. putida B-37 bacterial acdSgene expression. Expression of target gene exceeded reference gene in 1.27 times, those fact proved expression of acdS-gene in plants on high level. Expression of the heterologous gene in N. tabacum plants was also proved by biochemical method of ACC-deaminase specific activity define.