Open AccessDETERMINATION OF PHENOLIC, FLAVONOID, ANTIOXIDANT, AND ANTIBACTERIAL ACTIVITY OF MESEMBRYANTHEMUM CRYSTALLINMUM FROM LIBYAN COASTS
Author(s) -
Salmin Alshalmani,
Mudafara Bengleil,
Fatma Elshibani
Publication year - 2020
Publication title -
international journal of engineering applied science and technology
Language(s) - English
Resource type - Journals
ISSN - 2455-2143
DOI - 10.33564/ijeast.2020.v05i06.017
Subject(s) - flavonoid , antioxidant , traditional medicine , antibacterial activity , chemistry , biology , medicine , biochemistry , bacteria , genetics
The halophyte plant Mesembryanthemum crystallinum (family: Aizoaceae), widely used in the traditional medicine, was chosen for this study. There is no much information about the antioxidant activities of this plant growing in the East Libya coasts. The most of studies conducted confirmed the responses of this plant to the abiotic stresses. Methanolic extract was screened by applying general chemical tests for flavonoids, glycosides, reducing sugars, tannins, and saponins, proteins, and free amino acids. Preliminary phytochemical screening indicated the presence of phenolic compounds and flavonoids in the methanolic extracts of the plant. We demonstrate a high antioxidant activity in the methanolic extract of the plant by using the 1,1diphenyl-2-picrylhydrazyl (DPPH) method in concentration dependence manner. In term of antimicrobial activity the methanolic extract of plant revealed a broad spectrum activity against 3 strain of gram negative bacteria (Escherichia coli, Pseudomonas aeruginosa and Neisseria gonorrhoeae) and 3 strain of gram positive bacteria (Bacillus subtilis, Staphylococcus aureus and Streptococcus faecalis. The medicinal properties can be attributed in part to the presence of polyphenolics compounds in the plant extracts. Keywords— Mesembryanthemum crystallinumm, phenolic, flavonoid, antioxidant, and antimicrobial.