Open AccessIN-VITRO FREE RADICAL SCAVENGING AND ANTICANCER POTENTIAL OF METHANOL LEAF EXTRACT OF PLANT BACOPA MONNIERI AGAINST HCT15- CELL LINE
Author(s) -
Ramachandiran Mangaleshwari,
Muthiah Chandran
Publication year - 2020
Publication title -
international journal of engineering applied science and technology
Language(s) - English
Resource type - Journals
ISSN - 2455-2143
DOI - 10.33564/ijeast.2020.v05i04.046
Subject(s) - bacopa monnieri , in vitro , methanol , chemistry , cell culture , traditional medicine , botany , biology , biochemistry , medicine , organic chemistry , genetics
It is a universal fact that all living organisms are depending on plants for breathe, food, medicinal and everyday necessities. So, since the period of time immemorial, the peoples are using plant as a medicine to cure various diseases without knowing the scientific back-ground beyond this. The plant Bacopa monnieri (L.) Wettst, comes under the family Scrophulariaceae are commonly known by different names such as Water hyssop, Baby’s tear, Brahmi, Jalbrahmi, Nira-brahmi and Saraswati. In Ayurvedic system of ancient civilization, this plant is used to treat anxiety, improving cognitive functions, memory enhancement, hepato-protection and neuroprotection. This practice is continuing even today in the village side. Hence, the present study has been designed to evaluate the phytochemical constituents, extractive value to determine appropriate solvent to get maximum phytocompounds and antioxidant potential of the plant Bacopa monnieri. The obtained results have seen the presence of various important secondary metabolites such as amino acids, steroids, glycosides, flavonoids, alkaloids and tannins in methanolic extract. The Cytotoxicity analysis by the MTT (3-[4,5-dimethylthiazole-2-yl]-2,5diphenyltetrazolium bromide) assay method showed a significant decline in cell viability in the colon cancer cell line-HCT15 indicates the presence of anticancer activity for this plant extract. The anticancer potential of Bacopa monnieri increased with increasing concentrations of plant extract.