Open AccessGENETIC DIVERSITY ANALYSIS OF BRINJAL (SOLANUM MELONGENA L.) USING RAPD MARKERS
Author(s) -
Shetul Muna Parvin,
Golam Rabbani,
Abu Zafur Al Munsur
Publication year - 2020
Publication title -
international journal of engineering applied science and technology
Language(s) - English
Resource type - Journals
ISSN - 2455-2143
DOI - 10.33564/ijeast.2020.v05i03.082
Subject(s) - melongena , solanum , rapd , genetic diversity , biology , botany , horticulture , sociology , demography , population
An experiment was conducted at the Horticulture Farm, Department of Horticulture, Bangladesh Agricultural University, Mymensingh to study the variability of 12 selected accessions of brinjal using morphological traits and RAPD. Molecular characterization and diversity study was conducted using RAPD markers. Amplification with 5 decamer primers produced 54 RAPD markers of which 49 (89.56%) were polymorphic and 5 (10.44%) were monomorphic. The highest genetic distance (0.9445) and the lowest genetic identity (0.3889) were observed in the accessions SM59 vs. SM11 combination. The lowest genetic distance (0.1388) was observed between the accessions SM22 and SM29 and higher genetic identity was also observed in this combination. Dendrogram was constructed from genetic distance and 12 brinjal accessions fall in five clusters. Thus the RAPD analysis provided an effective method in investigating genetic variation and relatedness in twelve