Open AccessAge-Dependent Changes in the Function of Mitochondrial Membrane Permeability Transition Pore in Rat Liver Mitochondria
Author(s) -
René Endlicher,
Z Drahota,
Otto Kučera,
Z Červinková
Publication year - 2021
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.934734
Subject(s) - mitochondrial permeability transition pore , mitochondrion , mptp , calcium , membrane potential , chemistry , reactive oxygen species , endocrinology , inner mitochondrial membrane , medicine , depolarization , permeability (electromagnetism) , calcium metabolism , membrane permeability , homeostasis , microbiology and biotechnology , biology , biochemistry , programmed cell death , membrane , apoptosis , dopaminergic , dopamine
Mitochondria play an important role in the cell aging process.Changes in calcium homeostasis and/or increased reactiveoxygen species (ROS) production lead to the opening ofmitochondrial permeability transition pore (MPTP), depolarizationof the inner mitochondrial membrane, and decrease of ATPproduction. Our work aimed to monitor age-related changes inthe Ca2+ ion effect on MPTP and the ability of isolated rat livermitochondria to accumulate calcium. The mitochondrial calciumretention capacity (CRC) was found to be significantly affected bythe age of rats. Measurement of CRC values of the rat livermitochondria showed two periods when 3 to 17-week old ratswere tested. 3-week and 17-week old rats showed lower CRCvalues than 7-week old animals. Similar changes were observedwhile testing calcium-induced swelling of rat liver mitochondria.These findings indicate that the mitochondrial energy productionsystem is more resistant to calcium-induced MPTP openingaccompanied by the damaging effect of ROS in adult rats than inyoung and aged animals.