Open AccessPVA and PCL nanofibers are suitable for tissue covering and regeneration
Author(s) -
J Beznoska,
Jir̆ı́ Uhlı́k,
Andrea Kestlerová,
Martin Kralovič,
Radek Divín,
J Fedačko,
Jiří Beneš,
Milan J. Beneš,
Karolína Vocetková,
Věra Sovková,
Alois Nečas,
Andrea Nečasová,
J Holešovský,
Evžen Amler
Publication year - 2019
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.934389
Subject(s) - polyvinyl alcohol , nanofiber , regeneration (biology) , biomedical engineering , wound healing , skin repair , surgery , materials science , animal study , skin grafting , scaffold , grafting , medicine , composite material , biology , microbiology and biotechnology , polymer
The aim of the study was to evaluate the safety and efficacy of a new therapeutic approach to skin defects resulting from split thickness grafting. Within the study, nanofiber-based dressings fabricated using polyvinyl alcohol (PVA) and poly-ε-caprolactone (PCL) were used, with different mass density. The study was performed in 1 female minipig. Nine defects (approx. 4x4 cm) were made in the superficial skin layer. The tested materials were applied to the squared skin defect and covered by a Jelonet paraffin gauze, sutured in the corners of the defects. The animal was monitored daily during the healing process (21 days). On day 5, 12, and 27, the healing of the wound was evaluated, and a biopsy was performed for further histologic testing. At the end of the study (on day 27 after the procedure), the animal was euthanized, and a standard pathologic evaluation was performed. We can conclude that the nanofiber scaffold which was well tolerated, could be used as a smart skin cover which could be functionalized with another bioactive substances directly on the surgeon table, among potential bioactive substances belong platelet derivatives, antibiotics, etc.