Open AccessMolecular assessment of kidney allografts: are we closer to a daily routine?
Author(s) -
Andriy Trailin,
Petra Hrubá,
Ondřej Viklický
Publication year - 2020
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.934278
Subject(s) - molecular pathology , medicine , pathology , kidney transplantation , molecular diagnostics , kidney , transplantation , microarray , pathogenesis , intensive care medicine , bioinformatics , biology , gene , biochemistry , gene expression
Kidney allograft pathology assessment has been traditionally based on clinical and histological criteria. Despite improvements in Banff histological classification, the diagnostics in particular cases is problematic reflecting a complex pathogenesis of graft injuries. With the advent of molecular techniques, polymerase-chain reaction, oligo- and microarray technologies allowed to study molecular phenotypes of graft injuries, especially acute and chronic rejections. Moreover, development of the molecular microscope diagnostic system (MMDx) to assess kidney graft biopsies represents the first clinical application of a microarray-based method in transplantation. Whether MMDx may replace conventional pathology is the subject of ongoing research, however this platform is particularly useful in complex histological findings and may help clinicians to guide the therapy.