Open AccessVarious AKIP1 Expression Levels Affect Its Subcellular Localization but Have no Effect on NF-κB Activation
Author(s) -
Alena Keprová,
L. Kořínková,
Ivana Křížová,
Romana Hadravová,
Filip Kaufman,
Iva Pichová,
Tomáš Ruml,
Michaela Rumlová
Publication year - 2019
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.933961
Subject(s) - subcellular localization , microbiology and biotechnology , nucleus , transcription factor , hek 293 cells , cytosol , chemistry , protein subunit , cytoplasm , biology , gene , biochemistry , enzyme
A-kinase interacting protein 1 (AKIP1) has been shown to interact with a broad range of proteins involved in various cellular processes, including apoptosis, tumorigenesis, and oxidative stress suggesting it might have multiple cellular functions. In this study, we used an epitope-tagged AKIP1 and by combination of immunochemical approaches, microscopic methods and reporter assays we studied its properties. Here, we show that various levels of AKIP1 overexpression in HEK-293 cells affected not only its subcellular localization but also resulted in aggregation. While highly expressed AKIP1 accumulated in electron-dense aggregates both in the nucleus and cytosol, low expression of AKIP1 resulted in its localization within the nucleus as a free, non-aggregated protein. Even though AKIP1 was shown to interact with p65 subunit of NF-κB and activate this transcription factor, we did not observe any effect on NF-κB activation regardless of various AKIP1 expression level.