Open AccessAccurate normalization factor for wire myography of rat femoral artery
Author(s) -
Peter Slezák,
Iveta Waczulíková,
Peter Bališ,
Angelika Púzserová
Publication year - 2010
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.932043
Subject(s) - electrical impedance myography , myograph , femoral artery , normalization (sociology) , mesenteric arteries , circumference , anatomy , biomedical engineering , artery , chemistry , medicine , cardiology , mathematics , geometry , sociology , vasodilation , anthropology
Wire myograph is a device for the in vitro investigation of both,active and passive properties of arteries. Arteries from a varietyof animal species, pathological states, and vascular beds wereinvestigated using this method. We focus on the normalizationprocedure which is aimed to standardize experimental settingsand, in part, to simulate physiological conditions. Duringnormalization, it is determined the internal circumference of avessel stretched to a tension that corresponds to the transmuralpressure of 100 mm Hg (IC100). Once it is determined, theinternal circumference is traditionally set to (0.9⋅IC100).However, this constant 0.9, called also the normalization factor(NF), was experimentally determined for rat small mesentericarteries only. Therefore, the aim of our work was to show theinfluence of different NFs on the passive tension and reactivity ofboth, rat femoral arteries (FA) and the first branches of superiormesenteric arteries (MA). We found out that the maximal activewall tension of the FA was achieved at the NF value of 1.1, andthat of the MA at 0.9. Considering the values of the active walltension we suggest that higher reactivity and better signal-tonoise ratio in FA can be achieved when the NF is set at least to 1.0.