Open AccessExperimental methods for simultaneous measurement of action potentials and electrograms in isolated heart
Author(s) -
Jana Kolářová,
Kateřina Fialová,
Oto Janoušek,
Marie Novàkovâ,
Ivo Provazník
Publication year - 2010
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.932010
Subject(s) - optical mapping , impulse (physics) , artifact (error) , signal (programming language) , fluorescence , wavelength , biomedical engineering , noise (video) , chemistry , acoustics , optics , physics , computer science , artificial intelligence , cardiology , medicine , quantum mechanics , image (mathematics) , programming language
Monophasic action potential (MAP) can be recorded from theheart surface by optical method based on fluorescencemeasurement. The motion of isolated heart during experimentcaused additional noise in recorded signal. The motion artifactcan be eliminated by ratiometric fluorescence emissionmeasurements. This study is based on experiments in whichoptical MAP measurement is done by single-wavelength and dualwavelength measurement of fluorescence emission. Bothrecording setups are presented and their advantages anddisadvantages are discussed. MAPs recorded by both methodsfrom isolated rabbit hearts perfused according to Langendorff arepresented. Simultaneous electrograms (EG) and MAPs recordingare analyzed and measurement of velocity of impulse conductionthrough heart tissue is presented.