Open AccessMild hypothermia attenuates changes in respiratory system mechanics and modifies cytokine concentration in bronchoalveolar lavage fluid during low lung volume ventilation
Author(s) -
Pavel Dostál,
Marian Šenkeřík,
R Pařízková,
David Bareš,
Pavel Živný,
Helena Živná,
Viktor Černý
Publication year - 2010
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931877
Subject(s) - bronchoalveolar lavage , hypothermia , medicine , tidal volume , anesthesia , ventilation (architecture) , lung , positive end expiratory pressure , respiratory system , mechanical ventilation , respiratory physiology , mechanical engineering , engineering
Hypothermia was shown to attenuate ventilator-induced lunginjury due to large tidal volumes. It is unclear if the protectiveeffect of hypothermia is maintained under less injuriousmechanical ventilation in animals without previous lung injury.Tracheostomized rats were randomly allocated to non-ventilatedgroup (group C) or ventilated groups of normothermia (group N)and mild hypothermia (group H). After two hours of mechanicalventilation with inspiratory fraction of oxygen 1.0, respiratoryrate 60 min-1, tidal volume 10 ml·kg-1, positive end-expiratorypressure (PEEP) 2 cm H2O or immediately after tracheostomy innon-ventilated animals inspiratory pressures were recorded, ratswere sacrificed, pressure-volume (PV) curve of respiratorysystem constructed, bronchoalveolar lavage (BAL) fluid and aorticblood samples obtained. Group N animals exhibited a higher risein peak inspiratory pressures in comparison to group H animals.Shift of the PV curve to right, higher total protein and interleukin6 levels in BAL fluid were observed in normothermia animals incomparison with hypothermia animals and non-ventilatedcontrols. Tumor necrosis factor-α was lower in the hypothermiagroup in comparison with normothermia and non-ventilatedgroups. Mild hypothermia attenuated changes in respiratorysystem mechanics and modified cytokine concentration inbronchoalveolar lavage fluid during low lung volume ventilation inanimals without previous lung injury.