Open AccessHepcidin Expression in Adipose Tissue Increases during Cardiac Surgery
Author(s) -
Martin Vokurka,
Z Lacinová,
J Kremen,
P Kopecký,
J Bláha,
Květa Pelinková,
M Haluzík,
E Nečas
Publication year - 2010
Publication title -
physiological research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.647
H-Index - 70
eISSN - 1802-9973
pISSN - 0862-8408
DOI - 10.33549/physiolres.931759
Subject(s) - hepcidin , adipose tissue , ferroportin , inflammation , ferritin , medicine , endocrinology , transferrin receptor , serum iron , transferrin , white adipose tissue , anemia
Hepcidin, a key regulator of iron metabolism, plays a crucial rolein the pathogenesis of anemia of chronic disease. Although it isproduced mainly in the liver, its recently described expression inadipose tissue has been shown to be enhanced in massiveobesity due to chronic low-grade inflammation. Our objective wasto study the changes in hepcidin expression in adipose tissueduring acute-phase reaction. We measured hepcidin mRNAexpression from isolated subcutaneous and epicardial adiposetissue at the beginning and at the end of the surgery. Theexpression of mRNAs for hepcidin and other iron-related genes(transferrin receptor 1, divalent metal transporter 1, ferritin,ferroportin) were measured by real-time RT-PCR. Hepcidinexpression significantly increased at the end of the surgery insubcutaneous but not in epicardial adipose tissue. Apart from theincreased levels of cytokines, the parameters of iron metabolismshowed typical inflammation-induced changes. We suggest thatacute inflammatory changes could affect the regulation ofhepcidin expression in subcutaneous adipose tissue and thuspossibly contribute to inflammation-induced systemic changes ofiron metabolism.